Janghorbani M, Sundaresan A, Young V R
Clin Chim Acta. 1981 Jul 1;113(3):267-80. doi: 10.1016/0009-8981(81)90280-1.
A method based on Radiochemical Neutron Activation Analysis (RNAA) is described which allows simultaneous measurement of two stable isotopes of calcium, 46Ca and 48Ca, in human feces, plasma, and urine for the purpose of studying human nutrition and metabolism of calcium. It is shown that these measurements can be made with relative analytical precision of 1--5% depending on the particulars of a given experiment. The method has been applied in humans and data are given showing that kinetics of plasma appearance of 46 Ca administered orally with food can be readily investigated. This method allows investigation of a number of important nutritional and metabolic issues in all human population groups without regard to radioisotope safety considerations, and should prove especially helpful in relation to studies of calcium bioavailability from different foods in a variety of population groups for whom use of radiocalcium is not warranted.
本文描述了一种基于放射化学中子活化分析（RNAA）的方法，该方法可同时测量人粪便、血浆和尿液中钙的两种稳定同位素46Ca和48Ca，用于研究人体钙的营养和代谢。结果表明，根据特定实验的具体情况，这些测量的相对分析精度可达1%-5%。该方法已应用于人体，并给出了相关数据，表明口服随食物摄入的46Ca在血浆中出现的动力学可以很容易地进行研究。该方法无需考虑放射性同位素安全问题，就能对所有人群中的一些重要营养和代谢问题进行研究，对于那些无需使用放射性钙的不同人群中不同食物的钙生物利用度研究，该方法应会特别有用。
