Janghorbani M, Ting B T, Young V R
Am J Clin Nutr. 1981 Dec;34(12):2816-30. doi: 10.1093/ajcn/34.12.2816.
Use of stable isotopes of selenium in relation to enrichment of diets for studies of selenium absorption and metabolism in human subjects is discussed. A method based on radiochemical neutron activation analysis is described which allows accurate measurement of stable isotopes 74Se, 76Se, and 80Se in matrices (feces, plasma, red blood cells, and urine) of interest in metabolic studies. We show that these isotopes can routinely be measured with analytical precision and accuracy of 10% in samples of available size. This precision and accuracy is satisfactory for conduct of many nutritional experiments concerned with gastrointestinal absorption, plasma and red cell selenium turnover, and urinary excretion of the element, using an oral dose of 74Se. Original, experimental data are presented to illustrate the degree of enrichment of 74Se in feces, plasma, and urine after a single oral ingestion of 50 micrograms of the isotope with a breakfast meal in healthy young men.
本文讨论了在人类受试者硒吸收和代谢研究中，使用稳定同位素硒与膳食强化之间的关系。描述了一种基于放射化学中子活化分析的方法，该方法能够精确测量代谢研究中感兴趣的基质（粪便、血浆、红细胞和尿液）中的稳定同位素74Se、76Se和80Se。我们表明，在现有样本量下，这些同位素的常规测量分析精度和准确度可达10%。对于许多涉及胃肠道吸收、血浆和红细胞硒周转以及该元素尿排泄的营养实验，采用口服74Se剂量时，这种精度和准确度是令人满意的。本文给出了原始实验数据，以说明健康年轻男性早餐单次口服50微克该同位素后，粪便、血浆和尿液中74Se的富集程度。
