Kimura T, Kawashima S, Nishizuka Y
Endocrinol Jpn. 1980 Dec;27(6):739-45. doi: 10.1507/endocrj1954.27.739.
Female fetuses of C57BL/Tw mice were injected subcutaneously with 50 microgram 17 beta-estradiol on day 17 of fetal life. The treatment was proved to induce ovary-independent proliferation and cornification in the vaginal epithelium in later postnatal life. These fetuses were sacrificed on day 19 or 20, or on the 3rd day after birth. Histological observation on day 19 or 20 of fetal life did not show a particular effect of estrogen at any portion of the vaginal anlage, but at 3 days after birth, the epithelium of the Müllerian vagina in estrogen treated females consisted of a single layer of columnar cells, whereas that in controls had double-layered columnar cells. A study of the mitotic rate revealed that the epithelial cells of the Müllerian vagina as well as those of the cervix were dividing more actively in fetuses than in pups. Estrogen injection on day 17 induced a marked suppression of the mitotic rate in the epithelium of the Müllerian vagina and cervix on day 19 and 20. On the other hand, estrogen treatment caused a significant increase in the mitotic rate of the epithelium of the sinus vagina, at least on days 19 and 20. It was concluded that the epithelial cells of the sinus vagina respond positively to estrogen and thus possibly play the main role of the development of irreversible changes in the vagina.
在胎儿期第17天，给C57BL/Tw小鼠的雌性胎儿皮下注射50微克17β-雌二醇。已证实该处理可在出生后的后期生活中诱导阴道上皮发生不依赖卵巢的增殖和角化。这些胎儿在胎儿期第19天或20天，或出生后第3天被处死。在胎儿期第19天或20天进行的组织学观察未显示雌激素对阴道原基的任何部分有特殊作用，但在出生后3天，经雌激素处理的雌性小鼠的苗勒管阴道上皮由单层柱状细胞组成，而对照组的则为双层柱状细胞。对有丝分裂率的研究表明，苗勒管阴道以及子宫颈的上皮细胞在胎儿期比幼崽期分裂更活跃。在第17天注射雌激素可导致在第19天和20天苗勒管阴道和子宫颈上皮的有丝分裂率显著降低。另一方面，雌激素处理至少在第19天和20天导致窦道阴道上皮的有丝分裂率显著增加。得出的结论是，窦道阴道的上皮细胞对雌激素有积极反应，因此可能在阴道不可逆变化的发育中起主要作用。
