Suzuki Y, Arai Y
Anat Embryol (Berl). 1977 Oct 7;151(2):119-25. doi: 10.1007/BF00297475.
Pregnant ICR/JCL mice were treated with 6 mg of cyproterone acetate (CA) from days 14 to 20 of pregnancy to feminize male offspring. Feminized males delivered on day 20 of pregnancy by cesarean section were castrated the same day, injected with estradiol-17beta(E2) or sesame oil from the day of delivery (=day 1) to day 10 and sacrificed on day 60. In oil-injected feminized males, the vaginal epithelium was atrophic and did not show cornification. In feminized males given 20 microgram E2 neonatally, the vaginal epithelium exhibited well-differentiated stratified squamous organization, but was not cornified in seven out of the nine mice of this group. In the mice treated with 50 microgram E2, persistent cornification was recognized most frequently in the posterior two-thirds of the vaginal epithelium which is considered to originate from the urogenital sinus. However, the incidence of cornification in the anterior one-third which may contain the epithelial cells of müllerian duct was low. These results provide supporting evidence for the possible participation of epithelial cells which come from the urogenital sinus in the development of estrogen-independent persistent vaginal cornification in neonatally estrogenized mice.
在妊娠第14天至20天，给怀孕的ICR/JCL小鼠注射6毫克醋酸环丙孕酮（CA），以使雄性后代雌性化。在妊娠第20天通过剖宫产分娩的雌性化雄性小鼠在同一天进行去势，从分娩日（=第1天）至第10天注射17β-雌二醇（E2）或芝麻油，并在第60天处死。在注射油的雌性化雄性小鼠中，阴道上皮萎缩，未出现角化。在新生时给予20微克E2的雌性化雄性小鼠中，阴道上皮呈现出分化良好的复层鳞状组织结构，但该组9只小鼠中有7只未出现角化。在用50微克E2处理的小鼠中，在被认为起源于泌尿生殖窦的阴道上皮后三分之二区域最常观察到持续角化。然而，可能包含苗勒管上皮细胞的前三分之一区域的角化发生率较低。这些结果为泌尿生殖窦来源的上皮细胞可能参与新生期雌激素化小鼠中雌激素非依赖性持续性阴道角化的发生提供了支持证据。
