一种在Sephacryl S - 200超细微粒上纯化牛生长激素的简化程序。

A simplified procedure for purification of bovine growth hormone on Sephacryl S-200 superfine.

作者信息

Secchi C, Berrini A, Signorelli V, Biondi P A

出版信息

Ital J Biochem. 1981 Mar-Apr;30(2):117-26.

Abstract

The isolation and purification of bovine growth hormone from pituitary glands by a shorter and efficient sequence of stages which utilizes the Reichert approach (Reichert, 1975) is described. The major innovations are: 1) precipitation in 2 M ammonium sulfate at pH 7.9 instead of pH 7.0 to obtain the extract designated as C-2 by Reichert and 2) early gel-chromatography on Sephacryl S-200 of the C-2 extract. The bGH obtained has higher specific activity than the standard reference hormone NIH-GH-B16. Electrophoretic and size homogeneity is investigated by isoelectric focusing and sodium dodecyl sulfate electrophoresis.

摘要

本文描述了一种利用赖歇特方法（赖歇特，1975年），通过更短且高效的步骤从垂体中分离和纯化牛生长激素的方法。主要创新点包括：1）在pH 7.9而非pH 7.0的2M硫酸铵中沉淀，以获得赖歇特指定为C-2的提取物；2）对C-2提取物进行早期的Sephacryl S-200凝胶色谱分析。所获得的牛生长激素比标准参考激素NIH-GH-B16具有更高的比活性。通过等电聚焦和十二烷基硫酸钠电泳研究了其电泳和大小均一性。