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用于骨骼肌酸性磷酸酶测定的半透膜技术的定量组织化学研究。III. 一种改良的同步偶联技术。

Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. III. A modified simultaneous coupling technique.

作者信息

Stoward P J, Al-Sarraj B

出版信息

Histochemistry. 1981;71(4):599-607. doi: 10.1007/BF00508385.

DOI:10.1007/BF00508385
PMID:7263298
Abstract

Meijer's semipermeable membrane technique for acid phosphatase was modified by treating sections immediately after incubation with 70% ethanol for 30 min at room temperature instead of with formalin. The modification improved the validity of the technique considerably, by stabilising the specific final reaction product formed in presumed enzyme-containing sites. The modified technique thus seems promising for assaying the activity of acid phosphatase in sections of skeletal muscle.

摘要

梅杰氏酸性磷酸酶半透膜技术得到了改进，即在孵育后立即将切片在室温下用70%乙醇处理30分钟，而不是用福尔马林处理。通过稳定在假定含有酶的位点形成的特定最终反应产物，这种改进极大地提高了该技术的有效性。因此，这种改进后的技术在检测骨骼肌切片中酸性磷酸酶的活性方面似乎很有前景。

相似文献

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Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. III. A modified simultaneous coupling technique.用于骨骼肌酸性磷酸酶测定的半透膜技术的定量组织化学研究。III. 一种改良的同步偶联技术。
Histochemistry. 1981;71(4):599-607. doi: 10.1007/BF00508385.
2
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Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. I. Meijer's method.用于骨骼肌酸性磷酸酶测定的半透膜技术的定量组织化学研究。I. 梅杰氏法。
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引用本文的文献

1
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. II. Non-specific reactions in control sections.用于骨骼肌酸性磷酸酶测定的半透膜技术的定量组织化学研究。II. 对照切片中的非特异性反应。
Histochemistry. 1981;71(4):585-98. doi: 10.1007/BF00508384.
2
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. IV. A post-coupling technique.用于骨骼肌酸性磷酸酶测定的半透膜技术的定量组织化学研究。IV. 偶联后技术。
Histochemistry. 1982;74(3):367-77. doi: 10.1007/BF00493436.
3

本文引用的文献

1
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. II. Non-specific reactions in control sections.用于骨骼肌酸性磷酸酶测定的半透膜技术的定量组织化学研究。II. 对照切片中的非特异性反应。
Histochemistry. 1981;71(4):585-98. doi: 10.1007/BF00508384.
2
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. I. Meijer's method.用于骨骼肌酸性磷酸酶测定的半透膜技术的定量组织化学研究。I. 梅杰氏法。
Histochemistry. 1981;71(3):405-31. doi: 10.1007/BF00495883.
3
Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. I. Acid phosphatase.
Acid phosphatase activity of human gingival fibroblasts measured using a simultaneous coupling semi-permeable membrane technique.
采用同步偶联半透膜技术测定人牙龈成纤维细胞的酸性磷酸酶活性。
Histochem J. 1983 Aug;15(8):815-24. doi: 10.1007/BF01003344.
4
Quantitative cytochemistry of beta-galactosidase in normal and enzyme deficient (gal) pollen of Brassica campestris: application of the indigogenic method.甘蓝型油菜正常和酶缺陷（gal）花粉中β-半乳糖苷酶的定量细胞化学：靛蓝生成法的应用
Histochem J. 1984 Dec;16(12):1273-96. doi: 10.1007/BF01003726.
5
Acid phosphatase activity in soleus and plantaris muscle fibres of normal and dystrophic hamsters. A quantitative histochemical study.正常和营养不良仓鼠比目鱼肌与跖肌纤维中的酸性磷酸酶活性。一项定量组织化学研究。
Histochemistry. 1986;84(4-6):580-5. doi: 10.1007/BF00482995.
用于改善组织切片中酶活性组织化学显示的半透膜。I. 酸性磷酸酶
Histochemie. 1972;30(1):31-9. doi: 10.1007/BF00303934.
4
A cytochemical study of acid phosphatase in dystrophic hamster muscle.营养不良性仓鼠肌肉中酸性磷酸酶的细胞化学研究。
J Ultrastruct Res. 1977 Mar;58(3):219-34. doi: 10.1016/s0022-5320(77)90014-4.