Campbell J B, Stoward P J
Histochemistry. 1986;84(4-6):580-5. doi: 10.1007/BF00482995.
The activity of acid phosphatase in skeletal muscle fibres of the plantaris and soleus of normal and dystrophic male hamsters was quantified using a histochemical post-coupling semipermeable membrane technique. Although the absolute levels of activity were found to vary widely from one animal to another, the ratio of the mean activities in the two muscles in each animal was virtually constant. In normal muscles, the ratio was about 0.73 and in dystrophic muscles, about 0.77. The activity in plantaris muscle fibres was always significantly lower than that in the corresponding soleus fibres, and in normal fibres compared to dystrophic ones. Another difference was that in normal fibres the mean activity declined to a constant level in mature animals older than about 3 months. In contrast, the activity in dystrophic muscles appeared to fall exponentially throughout life. The functional significance of these findings is discussed.
采用组织化学后偶联半透膜技术对正常和营养不良雄性仓鼠的跖肌和比目鱼肌骨骼肌纤维中的酸性磷酸酶活性进行了定量分析。尽管发现不同动物之间的绝对活性水平差异很大，但每只动物的两块肌肉中的平均活性比值实际上是恒定的。在正常肌肉中，该比值约为0.73，在营养不良肌肉中约为0.77。跖肌纤维中的活性总是显著低于相应比目鱼肌纤维中的活性，并且与营养不良纤维相比，正常纤维中的活性也是如此。另一个差异是，在正常纤维中，平均活性在约3个月龄以上的成熟动物中下降至恒定水平。相比之下，营养不良肌肉中的活性在整个生命过程中似乎呈指数下降。讨论了这些发现的功能意义。
