Dorland L, van Halbeek H, Vleigenthart J F, Lis H, Sharon N
J Biol Chem. 1981 Aug 10;256(15):7708-11.
In an earlier report (Lis, H., and Sharon, N. (1978) J. Biol. Chem. 253, 3468-3476) it was concluded that the plant glycoprotein soybean agglutinin contains two types of N-glycosidically linked carbohydrate units, both consisting of mannose and N-acetylgucosamine, in a molar ratio of 9:2. Both were described to have the same core structure, Man alpha (1 leads to 6)[Man alpha (1 leads to 3)]Man beta (1 leads to 4)GlcNAc beta (1 leads to 4)GlcNAc, but different patterns of branching. Here we present data showing that the asparagine-linked carbohydrate moiety of soybean agglutinin is homogeneous and possesses the following structure: (formula, see text) This conclusion is based on high resolution 1H NMR spectroscopy at 500 MHz of the isolated glycopeptide, and at 360 MHz of the oligosaccharide Man9GlcNAc obtained after digestion of the crude soybean agglutinin glycopeptide by endo-beta-N-acetylglucosaminidase H. The revised structure is identical in all respects with that of the high mannose N-glycosidic units of porcine thyroglobulin, of bovine lactotransferrin, and of the glycoprotein from Chinese hamster ovary cell membranes.
在较早的一份报告中（利斯，H.，和沙龙，N.（1978年）《生物化学杂志》253卷，3468 - 3476页）得出结论，植物糖蛋白大豆凝集素含有两种类型的N - 糖苷键连接的碳水化合物单元，二者均由甘露糖和N - 乙酰葡糖胺组成，摩尔比为9 : 2。二者均被描述为具有相同的核心结构，即Manα(1→6)[Manα(1→3)]Manβ(1→4)GlcNAcβ(1→4)GlcNAc，但分支模式不同。在此我们提供的数据表明，大豆凝集素的天冬酰胺连接的碳水化合物部分是均一的，且具有以下结构：（分子式，见正文）这一结论基于对分离出的糖肽在500 MHz下以及对经内切β - N - 乙酰葡糖胺酶H消化粗大豆凝集素糖肽后得到的寡糖Man9GlcNAc在360 MHz下进行的高分辨率1H NMR光谱分析。修订后的结构在所有方面均与猪甲状腺球蛋白、牛乳铁蛋白以及中国仓鼠卵巢细胞膜糖蛋白的高甘露糖N - 糖苷单元的结构相同。
