Halle J C, Simonnin M P
J Biol Chem. 1981 Aug 25;256(16):8569-72.
A 1H NMR study of histidine hydrochloride dissolved in water/dimethyl sulfoxide mixtures gives evidence that dimethyl sulfoxide is able to induce a proton transfer from the imidazolinium nitrogens (mainly N3) to the carboxylate group when its mole fraction becomes greater than 42%. The new tautomeric equilibrium AH2++- in equilibrium AH2+ is quantitatively studied as a function of the solvent composition. In pure dimethyl sulfoxide, this equilibrium is strongly biased toward the monocation AH2+, which represents 91% of the total histidine hydrochloride.
