Wang A H, Yang C C
J Biol Chem. 1981 Sep 10;256(17):9279-82.
Orthorhombic crystals of cardiotoxin-analogue III from Taiwan cobra have been grown from 2-methyl-2,4-pentanediol solution. The space group is P222 with 2 molecules/asymmetric unit. Several different crystal forms of phospholipase A2 from Taiwan cobra have been produced with or without Ca2+ ion. One of them, grown from 30 mM sodium cacodylate buffer (pH 6 to 7) and 30% 2-methyl-2,4-pentanediol with 20 mg/ml of protein, diffracts well to 2 A resolution. It crystallizes in space group P3112 or P3212 with 2 molecules/asymmetric unit, but it can be related to a quasi-cubic space group P213.
台湾眼镜蛇心脏毒素类似物III的正交晶体已从2-甲基-2,4-戊二醇溶液中生长出来。空间群为P222，每个不对称单元有2个分子。台湾眼镜蛇磷脂酶A2在有或没有Ca2+离子的情况下产生了几种不同的晶体形式。其中一种是从30 mM二甲胂酸钠缓冲液（pH 6至7）和30% 2-甲基-2,4-戊二醇中生长出来的，蛋白质浓度为20 mg/ml，其衍射效果良好，分辨率可达2 Å。它在空间群P3112或P3212中结晶，每个不对称单元有2个分子，但它可以与准立方空间群P213相关。
