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用二硫苏糖醇测定布鲁氏菌免疫球蛋白G凝集抗体效价。

Determination of brucella immunoglobulin G agglutinating antibody titer with dithiothreitol.

作者信息

Klein G C, Behan K A

出版信息

J Clin Microbiol. 1981 Jul;14(1):24-5. doi: 10.1128/jcm.14.1.24-25.1981.

DOI:10.1128/jcm.14.1.24-25.1981

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271896/

Abstract

The routine brucella agglutination test measures both immunoglobulin M (IgM) and IgG brucella antibody titers; however, only an elevated IgG titer is significant for differentiating active from inactive disease in patients with symptoms lasting 3 or more weeks. The IgG antibody titer can be determined by treating the serum wih 2-mercaptoethanol to inactivate the IgM brucella antibodies while leaving the IgG brucella antibodies intact. Dithiothreitol, which also inactivates IgM, was compared with 2-mercaptoethanol for the determination of IgG brucella agglutination titers. The dithiothreitol and 2-mercaptoethanol test results agreed within +/- 1 dilution step in 103 of 105 serum specimens tested, for a 98.1% rate of agreement. The results indicate that dithiothreitol can be used in place of 2-mercaptoethanol for determining IgG brucella agglutination titers. Dithiothreitol does not have the offensive odor or the irritant properties of 2-mercaptoethanol.

摘要

常规布鲁氏菌凝集试验可检测免疫球蛋白M（IgM）和IgG布鲁氏菌抗体滴度；然而，对于症状持续3周或更长时间的患者，只有升高的IgG滴度对于区分活动性疾病和非活动性疾病才有意义。IgG抗体滴度可通过用2-巯基乙醇处理血清来测定，以使IgM布鲁氏菌抗体失活，同时保持IgG布鲁氏菌抗体完整。还将同样能使IgM失活的二硫苏糖醇与2-巯基乙醇用于测定IgG布鲁氏菌凝集滴度。在105份检测的血清标本中，103份的二硫苏糖醇和2-巯基乙醇检测结果在±1个稀释度范围内一致，一致率为98.1%。结果表明，二硫苏糖醇可用于替代2-巯基乙醇来测定IgG布鲁氏菌凝集滴度。二硫苏糖醇没有2-巯基乙醇的刺鼻气味或刺激性。