用于区分感染布鲁氏菌的牛和接种疫苗的牛的琼脂凝胶免疫扩散试验与其他血清学方法的比较
Comparison of an agar-gel immunodiffusion test with other serological methods for differentiating Brucella infected from vaccinated cattle.
作者信息
McMahon K J
出版信息
Can J Comp Med. 1983 Jan;47(1):86-7.
Abstract
An agar-gel immunodiffusion test was compared with other serological tests for detection of antibodies in the sera of 48 cattle vaccinated with Brucella abortus strain 19. Two hundred forty-two sera were tested over a 12 month period, and addition of positive reactions for each test resulted in totals of 170 for the card test, 74 for the standard tube-agglutination test, 71 for the 2-mercaptoethanol test, 64 for the dithiothreitol test and 22 for the agar-gel immunodiffusion test.
摘要
将琼脂凝胶免疫扩散试验与其他血清学试验进行比较，以检测48头接种布鲁氏菌19号菌株疫苗的牛血清中的抗体。在12个月的时间里对242份血清进行了检测，每次试验阳性反应的总和分别为：卡片试验170次、标准试管凝集试验74次、2-巯基乙醇试验71次、二硫苏糖醇试验64次、琼脂凝胶免疫扩散试验22次。
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Determination of brucella immunoglobulin G agglutinating antibody titer with dithiothreitol.用二硫苏糖醇测定布鲁氏菌免疫球蛋白G凝集抗体效价。
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Radial immunodiffusion test with a Brucella polysaccharide antigen for differentiating infected from vaccinated cattle.使用布鲁氏菌多糖抗原的径向免疫扩散试验，用于区分感染牛和接种疫苗的牛。
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