Donaldson D J, Dunlap M K
J Exp Zool. 1981 Jul;217(1):33-43. doi: 10.1002/jez.1402170105.
Epidermal closure of skin wounds on newt limb explants was inhibited to equal degrees by cytochalasins B,D and dihydrocytochalasin B (H2CB). The cytochalasin solvent, dimethylsulfoxide (DMSO), had no effect on migration at the low concentration present in the cytochalasin and control solutions. However, a 5% DMSO solution completely blocked mobility. Wounds on limb explants and limbs in situ responded similarly to cytochalasin treatment. Inhibition of migration by H2CB was reversible even when protein synthesis was reduced by 73%. Scanning electron microscopy of wound epithelium migrating on nucleopore filters revealed extensive lamellipodia on marginal cells and the first row of submarginal cells. Cytochalasin treatment produced plications in the upper surface and free edge of the normally smooth lamellipodia. This disturbance of the free edge revealed focal adhesions with the substratum. The fact that migration was inhibition by CD and H2CB (two cytochalasins with an affinity for contractile proteins but without some of the side effects of CB) leads us to conclude that epidermal cells utilize actin or actin-like proteins during wound closure. These results increase the likelihood that tissue cells of all types, whether in vitro or in vivo, share a common biochemical basis for cell movement.
细胞松弛素B、D和二氢细胞松弛素B（H2CB）对蝾螈肢体外植体皮肤伤口的表皮闭合具有同等程度的抑制作用。细胞松弛素的溶剂二甲基亚砜（DMSO），在细胞松弛素和对照溶液中存在的低浓度下对迁移没有影响。然而，5%的DMSO溶液完全阻断了迁移。肢体外植体上的伤口和原位肢体对细胞松弛素处理的反应相似。即使蛋白质合成减少73%，H2CB对迁移的抑制也是可逆的。在核孔滤膜上迁移的伤口上皮的扫描电子显微镜显示，边缘细胞和第一排亚边缘细胞上有广泛的片状伪足。细胞松弛素处理在正常光滑的片状伪足的上表面和自由边缘产生褶皱。自由边缘的这种干扰揭示了与基质的局部粘连。迁移受到CD和H2CB（两种对收缩蛋白有亲和力但没有CB的一些副作用的细胞松弛素）抑制这一事实使我们得出结论，表皮细胞在伤口闭合过程中利用肌动蛋白或肌动蛋白样蛋白。这些结果增加了所有类型的组织细胞，无论是在体外还是体内，在细胞运动方面具有共同生化基础的可能性。
