Everett R D
J Gen Virol. 1981 Jan;52(Pt 1):25-38. doi: 10.1099/0022-1317-52-1-25.
The replication of bacteriophage T5 DNA has been shown to proceed via branched concatemeric intermediates. The structures of this concatemeric DNA was studied with respect to single-stranded regions and single-strand interruptions by digestion with S1 nuclease and agarose gel electrophoresis after alkali denaturation. The results were compared with the pattern of 'nicks' in the mature virion DNA, and the possible origins of these nicks are discussed. The structure of T5 concatemeric DNA was also studied by electron microscopy. Replication forks, loops and rare circular structures were observed, all of which were similar to those seen in replicating DNA of other large phages. Phage capsid structures were detected in association with both concatemeric and mature phage length DNA. These observations are discussed in relation to the replication, maturation and packaging of T5 DNA.
已证明噬菌体T5 DNA的复制通过分支串联中间体进行。通过用S1核酸酶消化并在碱变性后进行琼脂糖凝胶电泳，研究了这种串联DNA的结构，涉及单链区域和单链中断。将结果与成熟病毒粒子DNA中的“切口”模式进行比较，并讨论了这些切口的可能起源。还通过电子显微镜研究了T5串联DNA的结构。观察到了复制叉、环和罕见的环状结构，所有这些都与在其他大型噬菌体的复制DNA中看到的结构相似。在串联和成熟噬菌体长度的DNA中都检测到了噬菌体衣壳结构。结合T5 DNA的复制、成熟和包装对这些观察结果进行了讨论。
