小鼠发育和衰老过程中的脑微管蛋白微异质性

Brain tubulin microheterogeneity in the mouse during development and aging.

作者信息

von Hungen K, Chin R C, Baxter C F

出版信息

J Neurochem. 1981 Aug;37(2):511-4. doi: 10.1111/j.1471-4159.1981.tb00485.x.

DOI:10.1111/j.1471-4159.1981.tb00485.x

Abstract

Mouse brain tubulin was analyzed on isoelectric focusing gels. High-resolution gels utilizing Bio-Rad ampholytes (pH 4--6) revealed 5--6 bands in the region corresponding to the alpha-subunit of tubulin and 10 or more for the beta-subunit. The same general banding pattern was observed regardless of the method of preparation of the tubulin. Two species prominent in the brains of immature mice, alpha 6 and beta 2, virtually disappeared during maturation, while species beta 6 to beta 10 appeared. No significant changes from the mature pattern were seen during aging (examined at 12, 23, and 30 months of age).

摘要

在等电聚焦凝胶上分析了小鼠脑微管蛋白。使用Bio-Rad两性电解质（pH 4-6）的高分辨率凝胶在对应于微管蛋白α亚基的区域显示出5-6条带，β亚基则有10条或更多条带。无论微管蛋白的制备方法如何，都观察到相同的一般条带模式。在未成熟小鼠脑中突出的两个种类，α6和β2，在成熟过程中几乎消失，而β6至β10种类出现。在衰老过程中（在12、23和30个月龄时检查）未观察到与成熟模式有显著变化。