Suzuki Y, Kamikawa T, Yamazaki N
Jpn Heart J. 1981 May;22(3):377-85. doi: 10.1536/ihj.22.377.
In order to evaluate the role of carnitine on fatty acid metabolism in subepicardial (Epi) and subendocardial (Endo) regions in ischemic heart, tissue levels of carnitine, free fatty acids (FFA), and adenosine triphosphate (ATP) were determined in ischemic, non-ischemic and border areas in dog hearts with acute regional ischemia. Acute regional ischemia was induced by ligation of the left anterior descending coronary artery for 15 min. In normal hearts, tissue carnitine levels were lower in Endo than in Epi. On the other hand, FFA levels were higher in Endo than in Epi. In ATP levels, no significant differences were observed between Endo and Epi. In acute regional ischemia, tissue carnitine levels decreased not only in ischemic and border areas but also in nonischemic area. And the levels were lower in Endo than in Epi in all areas. Tissue ATP levels were also lower in endo than in Epi in all areas. From nonischemic area toward the center of ischemic area, the difference in ATP levels between Endo and Epi became more prominent. Tissue FFA levels increased in ischemic and border areas, while no significant differences were observed between Endo and Epi. These results confirmed that Endo as metabolically more anaerobic than Epi even in normal heart and it became more prominent in the heart with acute regional ischemia.
为了评估肉碱在缺血性心脏的心外膜下（Epi）和心内膜下（Endo）区域脂肪酸代谢中的作用，在患有急性局部缺血的犬心脏的缺血区、非缺血区和边缘区测定了肉碱、游离脂肪酸（FFA）和三磷酸腺苷（ATP）的组织水平。通过结扎左冠状动脉前降支15分钟诱导急性局部缺血。在正常心脏中，Endo的组织肉碱水平低于Epi。另一方面，Endo的FFA水平高于Epi。在ATP水平方面，Endo和Epi之间未观察到显著差异。在急性局部缺血时，组织肉碱水平不仅在缺血区和边缘区降低，在非缺血区也降低。并且在所有区域，Endo的水平均低于Epi。在所有区域，Endo的组织ATP水平也低于Epi。从非缺血区向缺血区中心，Endo和Epi之间的ATP水平差异变得更加显著。缺血区和边缘区的组织FFA水平升高，而Endo和Epi之间未观察到显著差异。这些结果证实，即使在正常心脏中，Endo的代谢也比Epi更偏向无氧代谢，而在急性局部缺血的心脏中这种差异更加显著。
