左旋肉碱对缺血心脏中游离脂肪酸、酰基辅酶A和酰基肉碱组织水平的影响。

Effects of L-carnitine on tissue levels of free fatty acid, acyl CoA, and acylcarnitine in ischemic heart.

作者信息

Suzuki Y, Kamikawa T, Kobayashi A, Yamazaki N

出版信息

Adv Myocardiol. 1983;4:549-57. doi: 10.1007/978-1-4757-4441-5_53.

Abstract

In order to evaluate the protective effects of L-carnitine on ischemic myocardium, its effects on tissue levels of free fatty acid (FFA), acyl CoA, acyl carnitine, and adenosine triphosphate (ATP) were studied in ischemic dog hearts. Myocardial ischemia was induced by the ligation of left anterior descending coronary artery for 15 min. L-Carnitine (100 mg/kg) was administered intravenously prior to coronary ligation. In ischemic myocardium, tissue levels of free carnitine and ATP decreased, whereas long-chain acyl carnitine, long-chain acyl CoA, and FFA increased. Pretreatment of L-carnitine prevented the decrease in free carnitine and ATP and the increase in long-chain acyl carnitine and long-chain acyl CoA. A positive correlation was observed between ATP and free carnitine. On the other hand, a negative correlation was observed not only between ATP and the ratio of long-chain acyl CoA to free carnitine but also between ATP and the ratio of long-chain acyl carnitine to free carnitine. These results suggest that L-carnitine has protective effects on ischemic myocardium, probably by preventing the accumulation of long-chain acyl carnitine and long chain acyl CoA.

摘要

为了评估左旋肉碱对缺血心肌的保护作用，研究了其对缺血犬心脏组织中游离脂肪酸（FFA）、酰基辅酶A、酰基肉碱和三磷酸腺苷（ATP）水平的影响。通过结扎左冠状动脉前降支15分钟诱导心肌缺血。在冠状动脉结扎前静脉注射左旋肉碱（100mg/kg）。在缺血心肌中，游离肉碱和ATP的组织水平降低，而长链酰基肉碱、长链酰基辅酶A和FFA增加。左旋肉碱预处理可防止游离肉碱和ATP的降低以及长链酰基肉碱和长链酰基辅酶A的增加。观察到ATP与游离肉碱之间呈正相关。另一方面，不仅观察到ATP与长链酰基辅酶A与游离肉碱的比值之间呈负相关，而且ATP与长链酰基肉碱与游离肉碱的比值之间也呈负相关。这些结果表明，左旋肉碱对缺血心肌具有保护作用，可能是通过防止长链酰基肉碱和长链酰基辅酶A的积累来实现的。