一种用于比较微电泳实验中未标记药物迁移率的方法。

A procedure for comparing the mobilities of unlabeled drugs used in microelectrophoresis experiments.

作者信息

Bradshaw C M, Pun R Y, Slater N T, Szabadi E

出版信息

J Pharmacol Methods. 1981 Jan;5(1):67-73. doi: 10.1016/0160-5402(81)90104-2.

Abstract

A novel method for comparing the absolute mobilities of unlabeled compounds released from micropipettes in microelectrophoresis experiments is described. The method is based on the principle that the introduction of a "foreign" ion into an electrolyte reduces the transport number of a "reference" ion present in the electrolyte. Using [14C]-noradrenaline as the "reference" ion, the mobilities of two "foreign" ions, methoxamine and phenylephrine, were compared. No significant difference was found between the mobilities of the two drugs. It was concluded that the two drugs probably have similar transport numbers when released from solutions of equal molarity in microelectrophoresis experiments in vivo, and thus the previously reported difference between the apparent potencies of the two drugs is presumably of biological origin. The method described here may be of use in comparing the mobilities of other compounds, the radiolabeled forms of which are either unavailable or prohibitively expensive.

摘要

本文描述了一种在微电泳实验中比较从微量移液器释放的未标记化合物绝对迁移率的新方法。该方法基于这样一个原理：将“外来”离子引入电解质中会降低电解质中“参考”离子的迁移数。以[14C]-去甲肾上腺素作为“参考”离子，比较了两种“外来”离子甲氧明和去氧肾上腺素的迁移率。两种药物的迁移率之间未发现显著差异。得出的结论是，在体内微电泳实验中，当两种药物从等摩尔浓度的溶液中释放时，它们可能具有相似的迁移数，因此先前报道的两种药物表观效价之间的差异可能源于生物学原因。这里描述的方法可能有助于比较其他化合物的迁移率，这些化合物的放射性标记形式要么无法获得，要么价格高得令人望而却步。