The seed coats of field bean (Dolichos lablab var. lignosus) were found to contain bound hydroxyproline (0.26 g per 100 g dry weight) extractable in 30% with 5% trichloroacetic acid or in 90% with 10% potassium hydroxide. 2. The alkaline extract was fractionated by DEAE-cellulose chromatography into several hydroxyproline-containing fractions, composed of protein and carbohydrate in varying proportions. From the major fraction, a hydroxyproline-containing glycoprotein fraction was isolated by Sephadex G-200 chromatography. It gave a single band on agar-gel electrophoresis, and contained: protein (23.1%), arabinose (11.2%), galactose (13.4%), glucose (17.6%), mannose (22.6%) and uronic acids (11.3%). The content of hydroxyproline in the protein moiety was about 9%. Hydrolysis of the glycoprotein fraction with barium hydroxide yielded three components containing hydroxyproline and arabinose at the ratios of 1:2, 1:3 and 1:4.
摘要
发现长豇豆（Dolichos lablab var. lignosus）的种皮含有结合型羟脯氨酸（每100克干重含0.26克），可用30%的5%三氯乙酸提取，或用90%的10%氢氧化钾提取。2. 碱性提取物通过DEAE-纤维素色谱法分离成几个含羟脯氨酸的组分，这些组分由不同比例的蛋白质和碳水化合物组成。从主要组分中，通过Sephadex G-200色谱法分离出一个含羟脯氨酸的糖蛋白组分。它在琼脂凝胶电泳上呈现单一谱带，且含有：蛋白质（23.1%）、阿拉伯糖（11.2%）、半乳糖（13.4%）、葡萄糖（17.6%）、甘露糖（22.6%）和糖醛酸（11.3%）。蛋白质部分中羟脯氨酸的含量约为9%。用氢氧化钡水解糖蛋白组分产生了三种组分，其羟脯氨酸和阿拉伯糖的比例分别为1:2、1:3和1:4。
