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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Mani U V, Radhakrishnan A N

Biochem J. 1974 Jul;141(1):147-53. doi: 10.1042/bj1410147.

A hydroxyproline-containing protein was isolated from the soluble fraction of sandal leaves (Santalum album L.) and the purified protein was homogeneous by disc electrophoresis. 2. It is a glycoprotein containing 16% carbohydrate, the components of which were mainly arabinose, with only small amounts (about 5%) of galactose. The principal amino acids were glutamic acid, aspartic acid, glycine, alanine, arginine, lysine, proline and hydroxyproline, which together comprised 60% of the total. The number of acidic amino acids exceeds the number of basic amino acids. By Sephadex gel filtration, the approximate molecular weight was found to be about 63000. The ratio of residues of hydroxyproline to those of arabinose was 1:2. 3. The native protein is resistant to the action of several proteolytic enzymes. After partial hydrolysis with 0.1m-HCl, the protein became susceptible to attack by Pronase but remained resistant to collagenase.

从檀香叶（檀香属白檀）的可溶部分分离出一种含羟脯氨酸的蛋白质，经圆盘电泳分析，纯化后的蛋白质呈均一状态。2. 它是一种糖蛋白，含16%的碳水化合物，其成分主要是阿拉伯糖，只有少量（约5%）半乳糖。主要氨基酸为谷氨酸、天冬氨酸、甘氨酸、丙氨酸、精氨酸、赖氨酸、脯氨酸和羟脯氨酸，它们共占总量的60%。酸性氨基酸的数量超过碱性氨基酸。通过葡聚糖凝胶过滤法，发现其近似分子量约为63000。羟脯氨酸残基与阿拉伯糖残基的比例为1:2。3. 天然蛋白质对几种蛋白水解酶的作用具有抗性。用0.1m - HCl进行部分水解后，该蛋白质变得易受链霉蛋白酶攻击，但仍对胶原酶具有抗性。

Mani U V, Radhakrishnan A N

Biochem J. 1974 Jul;141(1):147-53. doi: 10.1042/bj1410147.

A hydroxyproline-containing protein was isolated from the soluble fraction of sandal leaves (Santalum album L.) and the purified protein was homogeneous by disc electrophoresis. 2. It is a glycoprotein containing 16% carbohydrate, the components of which were mainly arabinose, with only small amounts (about 5%) of galactose. The principal amino acids were glutamic acid, aspartic acid, glycine, alanine, arginine, lysine, proline and hydroxyproline, which together comprised 60% of the total. The number of acidic amino acids exceeds the number of basic amino acids. By Sephadex gel filtration, the approximate molecular weight was found to be about 63000. The ratio of residues of hydroxyproline to those of arabinose was 1:2. 3. The native protein is resistant to the action of several proteolytic enzymes. After partial hydrolysis with 0.1m-HCl, the protein became susceptible to attack by Pronase but remained resistant to collagenase.

从檀香叶（檀香属白檀）的可溶部分分离出一种含羟脯氨酸的蛋白质，经圆盘电泳分析，纯化后的蛋白质呈均一状态。2. 它是一种糖蛋白，含16%的碳水化合物，其成分主要是阿拉伯糖，只有少量（约5%）半乳糖。主要氨基酸为谷氨酸、天冬氨酸、甘氨酸、丙氨酸、精氨酸、赖氨酸、脯氨酸和羟脯氨酸，它们共占总量的60%。酸性氨基酸的数量超过碱性氨基酸。通过葡聚糖凝胶过滤法，发现其近似分子量约为63000。羟脯氨酸残基与阿拉伯糖残基的比例为1:2。3. 天然蛋白质对几种蛋白水解酶的作用具有抗性。用0.1m - HCl进行部分水解后，该蛋白质变得易受链霉蛋白酶攻击，但仍对胶原酶具有抗性。