Tomov T
Acta Physiol Pharmacol Bulg. 1978;4(1):62-8.
The effect of the functional state of isolated mitochondria on the fluorescence of pyronin added to the suspension is studied. It is shown experimentally that the intensity of fluorescence changes parallel to the degree of energization of the mitochondria. The magnitude and the sign of the fluorescent changes depend on the pyronin/mitochondria ratio and the length of the wave of excitating light. Model experiments show that the effects observed are probably due to the electrophoretic redistribution of pyronin between the medium, the mitochondrial membrane and the matrix, induced by the membrane potential of the mitochondria. It is assumed that pyronin acts as vector fluorescent indicator, similar to anilinonapthalenesulphonate.
研究了分离线粒体的功能状态对添加到悬浮液中的派洛宁荧光的影响。实验表明，荧光强度的变化与线粒体的能量化程度平行。荧光变化的幅度和符号取决于派洛宁/线粒体的比例以及激发光的波长。模型实验表明，观察到的效应可能是由于线粒体膜电位诱导派洛宁在介质、线粒体膜和基质之间的电泳再分布所致。假定派洛宁作为载体荧光指示剂，类似于苯胺萘磺酸盐。
