多头绒泡菌染色质的一些不寻常特征归因于黏液的存在。

Some unusual features of Physarum polycephalum chromatin are due to the presence of slime.

作者信息

Staroń K, Jerzmanowski A, Fronk J, Toczko K

出版信息

Acta Biochim Pol. 1980;27(3-4):413-20.

Abstract

Chromatin of lower eukaryote Physarum polycephalum, while showing typical nucleosomal organization, reveals upon digestion with micrococcal nuclease certain features not found in chromatins of higher eukaryotes, the most pronounced of which is the unusual pattern of degradation of core-size DNA, without accumulation of subcore fragments. It has been shown that these peculiarities are not due to intrinsic features of Physarum nucleohistone complex but to the presence of a specific polysaccharide, the main component of Physarum slime, contaminating chromatin preparations.

摘要

低等真核生物多头绒泡菌的染色质，虽然呈现典型的核小体结构，但在用微球菌核酸酶消化后，会展现出高等真核生物染色质中未发现的某些特征，其中最显著的是核心大小DNA的异常降解模式，且没有亚核心片段的积累。研究表明，这些特性并非源于多头绒泡菌核组蛋白复合物的内在特征，而是由于一种特定多糖的存在，这种多糖是多头绒泡菌黏液的主要成分，会污染染色体制备物。