Walliser S
Acta Biol Med Ger. 1981;40(2):161-5.
The alkylating agent Trenimon (10(-9) mol/l - 10(-6) mol/l) caused characteristic changes in membrane polarization, in cell counts and in surface charge (zeta potential). After short incubation time this effect of Trenimon is particularly pronounced in terms of transmembrane potential (TMP), whereas a decrease in surface charge was found only after incubation times longer than 24 hours. Immediately after addition of the chemotherapeutic agent to the HeLa cells damped oscillation of the TMP was observed with amplitudes being a function of the concentration of the agent. For incubation times between 24 and 96 hours low concentration (10(-8) mol/l) entailed increase in membrane polarization. Membrane depolarization was enhanced at higher doses of 10(-7) mol/l and 10(-6) mol/l. After lengthy incubation time drastic depolarizations and decrease in surface charge were accompanied by morphological cell damage such as "giant cells" and "off spherical" cells. Grunicke et al. [4] also found after Trenimon administration changes in membrane function before the DNA synthesis was influenced. These observations indicate that the cell membrane can be considered as a direct point for the effect of the alkylating agent Trenimon.
烷化剂三乙撑亚胺醌（10⁻⁹摩尔/升 - 10⁻⁶摩尔/升）可引起膜极化、细胞计数和表面电荷（ζ电位）的特征性变化。短时间孵育后，三乙撑亚胺醌的这种作用在跨膜电位（TMP）方面尤为明显，而表面电荷的减少仅在孵育时间超过24小时后才出现。将化疗药物加入HeLa细胞后，立即观察到TMP的阻尼振荡，其振幅是药物浓度的函数。在24至96小时的孵育时间内，低浓度（10⁻⁸摩尔/升）会导致膜极化增加。在10⁻⁷摩尔/升和10⁻⁶摩尔/升的较高剂量下，膜去极化增强。长时间孵育后，剧烈的去极化和表面电荷的减少伴随着细胞形态损伤，如“巨细胞”和“非球形”细胞。Grunicke等人[4]在给予三乙撑亚胺醌后也发现，在DNA合成受到影响之前，膜功能就发生了变化。这些观察结果表明，细胞膜可被视为烷化剂三乙撑亚胺醌作用的直接靶点。
