Walliser S, Redmann K
Arch Geschwulstforsch. 1981;51(1):125-32.
The transmembrane potential, the surface charge and the cell count were measured on cells cultured in vitro under the influence of various cytostatic substances. Lower concentrations of the agents induced an increase in membrane polarization. On the other hand membrane depolarization grew with both increasing exposure to cytostatic substances and rising content of the latter in the medium. The influence of higher doses was accompanied by morphological cell damage as well as a decrease in the surface charge of cells after a lengthy incubation time. Immediately after addition of chemotherapeutic substances to the cell cultures a damped oscillation of the TMP was observed. This clearly supports that the TMP is a rapidly reacting and very sensitive indicator for a great number of cell functions and an additional parameter in pretherapeutic sensitivity tests.
在各种细胞抑制物质的影响下，对体外培养的细胞进行跨膜电位、表面电荷和细胞计数的测量。较低浓度的这些物质会导致膜极化增加。另一方面，随着细胞抑制物质暴露时间的增加以及培养基中其含量的升高，膜去极化加剧。高剂量的影响伴随着细胞形态损伤以及长时间孵育后细胞表面电荷的减少。在向细胞培养物中添加化疗物质后，立即观察到跨膜电位出现衰减振荡。这清楚地表明跨膜电位是大量细胞功能的快速反应且非常敏感的指标，也是治疗前敏感性测试中的一个附加参数。
