Syversen T L, Totland G, Flood P R
Arch Toxicol. 1981 Apr;47(2):101-11. doi: 10.1007/BF00332352.
A single dose of 10 mg methylmercury chloride per kg body weight was given to 30 days old rats and to adult rats (180-200g)(. This resulted in brain levels of 1.4-2.2 micrograms Hg/g wet weight. In the young rats electron microscopic morphometry showed swelling of the granule cells. The extent of changes was more pronounced in the cerebellar hemispheres than in the vermis and flocculus. At 7 days after giving the methylmercury the granule cells appeared to have returned to normal. Methylmercury produced both light and electron microscopic changes in cerebellar neurons of adult (180-200 g) rats 3 days after dosing. 2.5-10% of the granule cells appeared dark and condensed in toluidine blue stained semithin sections of perfusion fixed and plastic embedded material. In control animals the comparable percentage never exceeded 1. By electron microscopic morphometry the dark cells proved to be shrunken to 70%, whereas the remaining light granule cells were swollen to 130% of the normal cell volume. The heterochromatin and mitochondrial volumes per cell remained constant in both dark and light cells from methylmercury treated animals. In the purkinje cells from both young and adult rats, geometrical changes in the cisternae of the granulated endoplasmic reticulum were evident. The swelling and shrinkage of the granule cells is supposed to be due to impaired electrolyte control and the disorganized granulated endoplasmic reticulum of the Purkinje cells may be related to the deleterious effect on protein synthesis.
给30日龄大鼠和成年大鼠（180 - 200克）每千克体重单次注射10毫克氯化甲基汞。这导致大脑中汞含量达到1.4 - 2.2微克/克湿重。在幼鼠中，电子显微镜形态计量学显示颗粒细胞肿胀。这种变化程度在小脑半球比在蚓部和绒球更明显。给予甲基汞7天后，颗粒细胞似乎已恢复正常。给药3天后，甲基汞在成年（180 - 200克）大鼠的小脑神经元中产生了光学和电子显微镜下的变化。在灌注固定和塑料包埋材料的甲苯胺蓝染色半薄切片中，2.5 - 10%的颗粒细胞显得暗黑且浓缩。在对照动物中，相应比例从未超过1%。通过电子显微镜形态计量学，暗黑细胞被证明缩小到正常细胞体积的70%，而其余明亮的颗粒细胞肿胀到正常细胞体积的130%。甲基汞处理动物的暗黑和明亮细胞中，每个细胞的异染色质和线粒体体积保持恒定。在幼鼠和成年大鼠的浦肯野细胞中，颗粒内质网池的几何形状变化明显。颗粒细胞的肿胀和收缩被认为是由于电解质控制受损，而浦肯野细胞颗粒内质网的紊乱可能与对蛋白质合成的有害影响有关。
