Severson D L, Fletcher T
Biochim Biophys Acta. 1981 Jul;675(2):256-64. doi: 10.1016/0304-4165(81)90235-x.
The regulation of acid cholesterol ester hydrolase activity by thyroid hormones was studied in subcellular fractions from rat liver, heart, and epididymal fat pads; hydrolase activity was determined at pH 5 with a glycerol-dispersed cholesterol oleate substrate preparation. Acid cholesterol ester hydrolase activity was decreased in liver preparations from thyroidectomized rats relative to activity in livers from euthyroid control rats. Administration of triiodothyronine to either euthyroid or hypothyroid (thyroidectomized) rats resulted in an increase in acid cholesterol ester hydrolase activity in liver preparations. Similar effects of thyroidectomy and the administration of triiodothyronine on acid cholesterol ester hydrolase activity were observed with fat pad preparations. In contrast, no effect of thyroid hormones was observed on acid cholesterol ester hydrolase activity in heart. These results suggest that thyroid hormones may regulate the catabolism of serum lipoproteins, in part, by alterations in lysosomal acid cholesterol ester hydrolase activity in liver and epididymal fat pads.
在大鼠肝脏、心脏和附睾脂肪垫的亚细胞组分中研究了甲状腺激素对酸性胆固醇酯水解酶活性的调节；使用甘油分散的胆固醇油酸酯底物制剂在pH 5下测定水解酶活性。相对于甲状腺功能正常的对照大鼠肝脏中的活性，甲状腺切除大鼠肝脏制剂中的酸性胆固醇酯水解酶活性降低。对甲状腺功能正常或甲状腺功能减退（甲状腺切除）的大鼠给予三碘甲状腺原氨酸，可导致肝脏制剂中酸性胆固醇酯水解酶活性增加。在脂肪垫制剂中观察到甲状腺切除和给予三碘甲状腺原氨酸对酸性胆固醇酯水解酶活性有类似影响。相比之下，未观察到甲状腺激素对心脏中酸性胆固醇酯水解酶活性有影响。这些结果表明，甲状腺激素可能部分通过改变肝脏和附睾脂肪垫中溶酶体酸性胆固醇酯水解酶的活性来调节血清脂蛋白的分解代谢。
