Hemminki K
Carcinogenesis. 1980 Apr;1(4):311-6. doi: 10.1093/carcin/1.4.311.
A fluorescence technique has been devised to identify guanosine adducts, substituted at the N7, O6 and N2 positions. The technique is based on fluorescence excitation spectra taken under neutral and alkaline conditions. At neutral pH, the N7- and O6- derivatives were fluorescent while at an alkaline pH the N7-derivatives were irreversibly destroyed with the loss of fluorescence. In alkaline solution the O6- and N2-derivatives were fluorescent but neutralization suppressed the fluorescence of the N2- derivatives. The technique was examined using methylmethanesulfonate, known to produce in the main N7- alkylation, and with diethyl sulfate known to produce N7- alkylation and to a lesser extent O6-alkylation. Fluorescence spectra typical of N 2-alkylation, in addition to N7-alkylation was produced by dimethylcarbamoylchloride.
已设计出一种荧光技术来鉴定在N7、O6和N2位置被取代的鸟苷加合物。该技术基于在中性和碱性条件下获得的荧光激发光谱。在中性pH值下，N7和O6衍生物具有荧光性，而在碱性pH值下，N7衍生物会不可逆地被破坏并失去荧光。在碱性溶液中，O6和N2衍生物具有荧光性，但中和作用会抑制N2衍生物的荧光。使用已知主要产生N7烷基化的甲磺酸甲酯以及已知产生N7烷基化且在较小程度上产生O6烷基化的硫酸二乙酯对该技术进行了检验。除了N7烷基化外，二甲基氨基甲酰氯还产生了典型的N2烷基化荧光光谱。
