Greiner J W, Evans C H, DiPaolo J A
Carcinogenesis. 1981;2(4):359-62. doi: 10.1093/carcin/2.4.359.
Chemical carcinogens or u.v. irradiation induce in vitro anchorage-independent growth and in vivo lethal multicellular infiltrative growth of human MRC-5 cells. A single carcinogen treatment 8 h after release of MRC-5 cells from metabolic block induced by a deficiency of arginine and glutamine in the medium is followed within 4--5 weeks by formation of colonies of greater than 50 cells in 0.35% semi-solid agar medium. Anchorage-independent MRC-5 cells, moreover, when injected intracranially into immunologically deficient homozygous nude mice, 6--7 weeks post-carcinogen exposure, produce progressive neurological dysfunction 5--6 weeks later accompanied by lethal multifocal multicellular infiltrating lesions. The present investigation demonstrates for the first time, carcinogen-induced anchorage-independent growth and in vivo lethality of a well characterized human diploid fibroblast cell strain and indicates the potential value of MRC-5 cell transformation as a new model for the study of carcinogenesis in human cells.
化学致癌物或紫外线照射可在体外诱导人MRC-5细胞的锚定非依赖性生长，并在体内诱导其致死性多细胞浸润性生长。在培养基中缺乏精氨酸和谷氨酰胺导致MRC-5细胞代谢阻断后8小时进行单次致癌物处理，4至5周内，在0.35%半固体琼脂培养基中会形成大于50个细胞的集落。此外，在致癌物暴露6至7周后，将锚定非依赖性MRC-5细胞颅内注射到免疫缺陷的纯合裸鼠中，5至6周后会产生进行性神经功能障碍，并伴有致死性多灶性多细胞浸润性病变。本研究首次证明了致癌物诱导的一种特征明确的人二倍体成纤维细胞系的锚定非依赖性生长和体内致死性，并表明MRC-5细胞转化作为研究人类细胞癌变的新模型具有潜在价值。
