Lacorre-Arescaldino I, Toublan B, Pastisson C
Faculté des Sciences, Laboratoire de Biologie Cellulaire, B.P. 347, 51062, Reims Cédex, France.
Rouxs Arch Dev Biol. 1988 Mar;197(2):124-127. doi: 10.1007/BF00375935.
Using flow cytometry, spherulating nuclei of Physarum isolated at the beginning of spherule wall formation were found to exhibit a DNA content corresponding to the G phase of the cell cycle, although 8% lower. Before the first mitosis after spherule germination, a very slight incorporation of H thymidine into DNA was observed that was too weak to correspond to S phase, strongly suggesting that nuclei are stopped in G phase inside the spherules. The lower value of nuclear DNA content found using flow cytometry of germinating spherules may not be related to DNA quantity, but may be due to a difference in chromatin organization during growth or spherulation, resulting in interference with the staining.
利用流式细胞术发现，在液泡壁形成开始时分离得到的绒泡菌正在形成液泡的细胞核，其DNA含量对应于细胞周期的G期，不过低了8%。在液泡萌发后的第一次有丝分裂之前，观察到有非常少量的H胸腺嘧啶核苷掺入DNA，但其微弱程度不足以对应于S期，这强烈表明细胞核在液泡内停滞于G期。利用流式细胞术对萌发中的液泡进行检测时发现的较低细胞核DNA含量值，可能与DNA数量无关，而可能是由于生长或形成液泡过程中染色质组织的差异，导致对染色产生干扰。
