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流式细胞术在定量实验性肿瘤对辐射反应中的应用。

The application of flow cytometry for the quantification of the response of experimental tumors to irradiation.

作者信息

Beck H P, Brammer I, Zywietz F, Jung H

出版信息

Cytometry. 1981 Jul;2(1):44-6. doi: 10.1002/cyto.990020110.

DOI:10.1002/cyto.990020110
PMID:7273976
Abstract

R1H rhabdomyosarcomas of the rat were irradiated with 15 Gy of x-rays and the depopulation and repopulation of the tumor was determined by means of flow cytometry, quantitative histology and colony assays. The results show that flow cytometry yields important information about the cell population kinetics of irradiated tumors, but misleading results are obtained if flow cytometry is not combined with the determination of the numerical density of cells.

摘要

用15戈瑞的X射线照射大鼠的R1H横纹肌肉瘤,通过流式细胞术、定量组织学和集落分析来确定肿瘤的细胞减少和再增殖情况。结果表明,流式细胞术可提供有关受照射肿瘤细胞群体动力学的重要信息,但如果不结合细胞数密度的测定,则会得出误导性结果。

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