Baumann M, Würschmidt F, Twardy A, Beck-Bornholdt H P
Department of Radiation Therapy, University Hospital Eppendorf, Hamburg, Germany.
Radiat Res. 1994 Dec;140(3):432-6.
The impact of intratumoral stroma on the expression of the tumor bed effect was studied in R1H rat rhabdomyosarcoma. For this, either R1H tumors growing in subcutaneous tissues (in situ) were irradiated at median volumes of 40 mm3 with a single exposure of 15 Gy (200 kVp X rays), or fresh tumor fragments or cloned tumor cells cultured in vitro were transplanted into subcutaneous tissue 2 days after preirradiation with 15 Gy. The tumor bed effect was evaluated by the time necessary for experimental tumors to grow from 0.1 cm3 to 10 cm3. When irradiated in situ, R1H tumors expressed a clear-cut tumor bed effect. The growth rate was decreased by a factor of 1.8 +/- 0.2 compared to controls. A significant decrease in growth rate by a factor of 1.7 +/- 0.2 was also observed when cultured tumor cells were transplanted into preirradiated tissue. However, when tumor fragments were transplanted into preirradiated sites, no significant decrease in growth rate occurred in two independent experiments (1.0 +/- 0.1; 1.2 +/- 0.2). Our results indicate that vessels originating from the intratumoral stroma can modulate the expression of the tumor bed effect in R1H tumors.
在R1H大鼠横纹肌肉瘤中研究了瘤内基质对肿瘤床效应表达的影响。为此，将生长于皮下组织（原位）的R1H肿瘤以40 mm3的中位体积单次照射15 Gy（200 kVp X射线），或者将体外培养的新鲜肿瘤碎片或克隆肿瘤细胞在15 Gy预照射2天后移植到皮下组织中。通过实验肿瘤从0.1 cm3生长至10 cm3所需的时间来评估肿瘤床效应。当原位照射时，R1H肿瘤表现出明显的肿瘤床效应。与对照组相比，生长速率降低了1.8±0.2倍。当将培养的肿瘤细胞移植到预照射组织中时，也观察到生长速率显著降低，降低了1.7±0.2倍。然而，当将肿瘤碎片移植到预照射部位时，在两项独立实验中未观察到生长速率显著降低（1.0±0.1；1.2±0.2）。我们的结果表明，源自瘤内基质的血管可以调节R1H肿瘤中肿瘤床效应的表达。
