Kimura H, Yamaguchi Y
Gan. 1981 Feb;72(1):72-80.
The distribution of in vitro-stimulated immune lymphocytes in tumor-bearing mice was studied. Spleen cells from BALB/c mice which had regressed Moloney murine sarcoma virus (M-MSV)-induced primary tumors, were sensitized in vitro by using Moloney murine leukemia virus-induced BALB/c lymphoma (LSTRA). Lymphocytes obtained 6 days after stimulation were examined for cytotoxic activity against LSTRA cells, labeled with 51 Cr or 99mTc, and inoculated into mice bearing MSv-induced primary tumors. The distribution of 52Cr-labeled lymphocytes was determined by counting the radioactivity of each organ. Compared to normal lymphocytes, in vitro-stimulated lymphocytes accumulated significantly in tumor tissues and lymphatic organs. The accumulation of MSV-immune lymphocytes in tumor tissues was not evident in 3-methylcholanthrene-induced BALB/c fibrosarcoma, suggesting the operation of specific mechanisms of accumulation of immune lymphocytes. Scintigraphy was performed by inoculating the 99mTc-labeled lymphocytes via the tail vein the tumor-bearing mice. Visualization of the tumor was possible in mice given in vitro-stimulated lymphocytes.
研究了荷瘤小鼠体内体外刺激的免疫淋巴细胞的分布。取已消退莫洛尼氏鼠肉瘤病毒（M-MSV）诱导的原发性肿瘤的BALB/c小鼠的脾细胞，用莫洛尼氏鼠白血病病毒诱导的BALB/c淋巴瘤（LSTRA）在体外进行致敏。刺激6天后获得的淋巴细胞，检测其对用51Cr或99mTc标记的LSTRA细胞的细胞毒性活性，并接种到荷MSv诱导原发性肿瘤的小鼠体内。通过计数各器官的放射性来确定52Cr标记淋巴细胞的分布。与正常淋巴细胞相比，体外刺激的淋巴细胞在肿瘤组织和淋巴器官中显著聚集。在3-甲基胆蒽诱导的BALB/c纤维肉瘤中，MSV免疫淋巴细胞在肿瘤组织中的聚集不明显，提示存在免疫淋巴细胞聚集的特异性机制。通过经尾静脉给荷瘤小鼠接种99mTc标记的淋巴细胞进行闪烁扫描。给体外刺激淋巴细胞的小鼠可实现肿瘤可视化。
