Kimura H
Gan. 1978 Aug;69(4):507-15.
The effect of cryosurgery and surgical operation on Moloney murine sarcoma virus-induced primary tumors in the treated and untreated groups of BALB/c mice was compared in terms of tumor growth, cumulative mortality, lymphocyte-mediated cytotoxicity, proliferative response of lymphocytes, and humoral antibody formation. The results indicate that cryosurgical treatment showed both in vivo and in vitro effects; (i) tumor growth and cumulative mortality in mice treated by cryosurgery were significantly lower than those in untreated groups, and (ii) their cellular immune response was enhanced, as manifested by increase in a proliferative response and in lymphocyte-mediated cytotoxic activity against Moloney murine leukemia virus-induced lymphoma. The proliferative response of spleen cells and the cytotoxic activity of lymphocytes were not parallel; the proliferative response detected by 3H-thymidine incoroporation manifested peak activity 3 days after cryosurgery, but the cytotoxic activity detected by 125I-iododeoxyuridine-releasing tests was considerably decreased at this stage. Two weeks after cryosurgery and thereafter, however, the cytotoxic activity of this group increased to a level higher than that of untreated or surgically treated groups. On the contrary, surgical operation abrogated the proliferative response of spleen cells. Immunofluorescence tests revealed, however, that humoral antibody formation was higher in surgically operated groups than in other groups.
比较了冷冻手术和外科手术对BALB/c小鼠莫洛尼氏鼠肉瘤病毒诱导的原发性肿瘤的影响，这些小鼠被分为治疗组和未治疗组，比较指标包括肿瘤生长、累积死亡率、淋巴细胞介导的细胞毒性、淋巴细胞的增殖反应以及体液抗体形成。结果表明，冷冻手术治疗显示出体内和体外效应；（i）冷冻手术治疗的小鼠的肿瘤生长和累积死亡率显著低于未治疗组，并且（ii）它们的细胞免疫反应增强，表现为增殖反应增加以及针对莫洛尼氏鼠白血病病毒诱导的淋巴瘤的淋巴细胞介导的细胞毒性活性增加。脾细胞的增殖反应和淋巴细胞的细胞毒性活性并不平行；通过3H-胸腺嘧啶核苷掺入检测到的增殖反应在冷冻手术后3天表现出峰值活性，但在此阶段通过125I-碘脱氧尿苷释放试验检测到的细胞毒性活性却大幅下降。然而，在冷冻手术后两周及之后，该组的细胞毒性活性增加到高于未治疗组或手术治疗组的水平。相反，外科手术消除了脾细胞的增殖反应。然而，免疫荧光试验显示，手术治疗组的体液抗体形成高于其他组。
