Bacellar O, Brodskyn C, Guerreiro J, Barral-Netto M, Costa C H, Coffman R L, Johnson W D, Carvalho E M
Immunology Service of Hospital Universitário Professor Edgard Santos, Federal University of Bahia, Brazil.
J Infect Dis. 1996 Jun;173(6):1515-8. doi: 10.1093/infdis/173.6.1515.
American visceral leishmaniasis (AVL) is associated with the absence of lymphocyte proliferative responses and interleukin (IL)-2 and interferon-gamma (IFN-gamma) production upon stimulation with Leishmania antigen. In contrast, cure of AVL is associated with restoration of these T cell functions. In the present study, the ability of IL-12, a cytokine that acts on NK and T cells to restore cellular immune responses in AVL, was evaluated. Participants of the study included 12 patients with AVL and 7 subjects cured of AVL. The [3H]thymidine uptake and IFN-gamma production in cultures of peripheral blood mononuclear cells (from AVL patients) stimulated with Leishmania chagasi antigen were 882 +/- 1393 cpm and zero, respectively. Addition of IL-12 enhanced the proliferative response to 5097 +/- 6429 cpm (P < .001) and IFN-gamma production to 305 +/- 325 pg/mL (P < .01). IL-12 also restored cytotoxic activity against the K562 cell line. These results indicate that IL-12 has an important role in the regulation of the cellular immune response in human leishmaniasis.
美洲内脏利什曼病（AVL）与利什曼原虫抗原刺激后淋巴细胞增殖反应缺失以及白细胞介素（IL）-2和干扰素-γ（IFN-γ）产生缺乏有关。相比之下，AVL的治愈与这些T细胞功能的恢复相关。在本研究中，评估了白细胞介素-12（一种作用于自然杀伤细胞和T细胞的细胞因子）恢复AVL患者细胞免疫反应的能力。该研究的参与者包括12例AVL患者和7例已治愈的AVL患者。用恰加斯利什曼原虫抗原刺激外周血单个核细胞（来自AVL患者）培养物中的[3H]胸腺嘧啶核苷摄取量和IFN-γ产生量分别为882±1393 cpm和零。添加IL-12后，增殖反应增强至5097±6429 cpm（P<.001），IFN-γ产生量增加至305±325 pg/mL（P<.01）。IL-12还恢复了对K562细胞系的细胞毒性活性。这些结果表明，IL-12在人类利什曼病细胞免疫反应的调节中具有重要作用。
