Ohshima K
Invest Urol. 1981 Sep;19(2):79-84.
Effects of various osmotic solutions on membrane properties of smooth muscle cells of the guinea pig ureter were investigated using the microelectrode and double sucrose gap methods. In Krebs solution, the mean membrane potential was -53 mV, chronaxie was 104 msec, length constant of the tissue was 1.03 mm, time constant of the membrane was 65.8 msec, and conduction velocity of excitation was 19.2 mm per sec. Hyperosmotic solution (1.5, 2.0, or 2.5 times the normal osmolarity) depolarized the membrane, generated the spike activity, reduced the length constant of the tissue, increased the time constant of the membrane, prolonged the chronaxie, and reduced the conduction velocity. Hyposmotic solutions (0.85 and 0.67 times the normal osmolarity) produced opposite changes on the passive and active characteristics of the membrane compared to findings in hyperosmotic solution except that the time constant of the membrane was increased in both hyper- and hyposmolar solutions. Increase in the time constant of the membrane and reduced length constant of the tissue in hyperosmotic solution can be explained by an increase in the internal resistance, including the cell to cell junctional resistance and shrinkage of the cell diameter (from 6.2 to 3.0 micrometer).
采用微电极和双蔗糖间隙法研究了不同渗透压溶液对豚鼠输尿管平滑肌细胞膜特性的影响。在Krebs溶液中，平均膜电位为-53 mV，时值为104毫秒，组织长度常数为1.03毫米，膜时间常数为65.8毫秒，兴奋传导速度为每秒19.2毫米。高渗溶液（正常渗透压的1.5、2.0或2.5倍）使膜去极化，产生锋电位活动，减小组织长度常数，增加膜时间常数，延长时值，并降低传导速度。低渗溶液（正常渗透压的0.85和0.67倍）与高渗溶液相比，对膜的被动和主动特性产生相反的变化，但高渗和低渗溶液中膜时间常数均增加。高渗溶液中膜时间常数增加和组织长度常数减小可通过内阻增加来解释，包括细胞间连接电阻增加和细胞直径缩小（从6.2微米至3.0微米）。
