Dahl D, Bignami A
J Comp Neurol. 1977 Dec 15;176(4):645-57. doi: 10.1002/cne.901760412.
Antigens isolated by hydroxyapatite chromatography from human sciatic nerve (SN1 protein) and from 8 M urea extracts of chicken brain were selectively localized by immunofluorescence to neurofibrils in rat and chicken CNS. Absorption of the antisera with SN1 protein, chicken antigen or GFA protein abolished the staining. Antisera raised against antigen isolated with the same procedure from buffer extracts of chicken brain stained both neurofibrils and glial fibrils by immunofluorescence. Neurofibrillary staining was selectively abolished by absorption of the antisera with SN1 protein. Antisera prepared against axonal preparations isolated from bovine white matter only stained astroglia and were thus undistinguishable from anti-GFA sera in this respect. The data suggested that the protein subunits of neurofilament and glial filaments, although difficult to separate in brain extracts by standard biochemical procedures and by subcellular fractionation in bovine white matter, still retain immunological specificity. In addition, the immunological cross reactivity between human and chicken antigens suggested that neurofilaments, as other constituents of the cytoskeleton such as microtubules and actin microfilaments, show a high degree of evolutionary stability.
通过羟基磷灰石层析从人坐骨神经中分离出的抗原（SN1蛋白）以及从鸡脑的8M尿素提取物中分离出的抗原，经免疫荧光法选择性定位于大鼠和鸡中枢神经系统的神经原纤维。用SN1蛋白、鸡抗原或胶质纤维酸性蛋白（GFA蛋白）吸收抗血清后，染色消失。用相同方法从鸡脑缓冲提取物中分离出抗原后制备的抗血清，经免疫荧光法可使神经原纤维和胶质原纤维都染色。用SN1蛋白吸收抗血清后，神经原纤维染色选择性消失。针对从牛白质中分离出的轴突制剂制备的抗血清仅使星形胶质细胞染色，因此在这方面与抗GFA血清无法区分。数据表明，神经丝和胶质丝的蛋白质亚基，尽管通过标准生化程序以及在牛白质中进行亚细胞分级分离很难在脑提取物中分离，但仍保留免疫特异性。此外，人和鸡抗原之间的免疫交叉反应表明，神经丝与细胞骨架的其他成分如微管和肌动蛋白微丝一样，具有高度的进化稳定性。
