Dalsgaard C J, Björklund H, Jonsson C E, Hermansson A, Dahl D
Histochemistry. 1984;81(2):111-4. doi: 10.1007/BF00490102.
Neurofilament immunoreactive nerve fibers were demonstrated in human skin using indirect immunohistochemical technique with antibodies to neurofilament polypeptides. Neurofilament-positive fibers were seen as free nerve endings in the epidermis and in dermal papilla, in Meissner's corpuscles and as fibers crossing in the dermis. Strongly fluorescent nerve fibers were also seen around hair follicles, sweat gland ducts and sometimes in relation to blood vessels. From the distribution pattern it was concluded that predominantly sensory nerve fibers were labelled and that this technique may be used to study reinnervation of cutaneous sensory nerves following traumatic injuries and surgical procedures.
利用针对神经丝多肽的抗体，采用间接免疫组织化学技术在人体皮肤中证实了神经丝免疫反应性神经纤维。神经丝阳性纤维在表皮和真皮乳头中表现为游离神经末梢，在梅克尔小体中也有，并且在真皮中呈交叉状纤维。在毛囊、汗腺导管周围也可见强荧光神经纤维，有时还与血管有关。从分布模式可以得出结论，主要标记的是感觉神经纤维，并且该技术可用于研究创伤性损伤和外科手术后皮肤感觉神经的再支配情况。
