Saria A, Lembeck F, Skofitsch G
J Chromatogr. 1981 Apr 3;208(1):41-6. doi: 10.1016/s0021-9673(00)87957-1.
Capsaicin, the pungent principle of chili peppers, and six analogues were characterized by reversed-phase high-performance liquid chromatography using isocratic methanol--water mixtures and UV detection. A highly sensitive method for the quantitative determination of capsaicin in animal tissues and blood has been developed. Using a reversed-phase column and acetonitrile--water mixtures for elution, 3 ng of capsaicin could be measured by fluorimetric detection at an excitation wavelength of 270 nm and an emission wavelength of 330 nm. Acetone extraction of tissue resulted in a recovery of approximately 90%. Extraction and determination of capsaicin in rat brain, spinal cord, liver and blood after i.v. administration is given as an example.
辣椒素是辣椒的辛辣成分，采用等度甲醇 - 水混合物和紫外检测的反相高效液相色谱法对其及六种类似物进行了表征。已开发出一种用于定量测定动物组织和血液中辣椒素的高灵敏度方法。使用反相柱和乙腈 - 水混合物进行洗脱，通过在激发波长270 nm和发射波长330 nm处的荧光检测可测定3 ng辣椒素。组织的丙酮萃取回收率约为90%。以静脉注射给药后大鼠脑、脊髓、肝脏和血液中辣椒素的萃取和测定为例。
