Kawada T, Watanabe T, Katsura K, Takami H, Iwai K
J Chromatogr. 1985 Jul 5;329(1):99-105. doi: 10.1016/s0021-9673(01)81899-9.
A new, highly sensitive method for quantitative separation and determination of capsaicin and dihydrocapsaicin by high-performance liquid chromatography with electrochemical detection is elaborated. The method employs a Cosmosil 5Ph column eluted with 100 mM potassium dihydrogenphosphate containing 45% acetonitrile (pH 5.0) at a flow-rate of 1.0 ml/min, and an electrochemical detector (at a potential of +750 mV versus the Ag/AgCl electrode). The detection limits for both capsaicin and dihydrocapsaicin were 12 pg (39 fmol) at a signal-to-noise ratio of 3:1. By applying this method, the biological half-life of capsaicin in the rat was investigated.
阐述了一种采用高效液相色谱-电化学检测法定量分离和测定辣椒素和二氢辣椒素的高灵敏度新方法。该方法使用Cosmosil 5Ph柱，以含45%乙腈的100 mM磷酸二氢钾（pH 5.0）为流动相，流速为1.0 ml/min，并使用电化学检测器（相对于Ag/AgCl电极，电位为+750 mV）。在信噪比为3:1时，辣椒素和二氢辣椒素的检测限均为12 pg（39 fmol）。应用该方法研究了辣椒素在大鼠体内的生物半衰期。
