Holder A T, Preece M A, Wallis M
J Endocrinol. 1981 Sep;90(3):411-20. doi: 10.1677/joe.0.0900411.
Serum from adrenalectomized rats was equipotent with serum from non-adrenalectomized animals when measured in a rat cartilage somatomedin bioassay. Extraction with butanol of sera from normal or adrenalectomized rats reduced their potency in the somatomedin bioassay rather than increasing it as has been previously reported. Butanol-soluble inhibitors of cartilage metabolism were found in sera from both normal and adrenalectomized rats. Cortisol and corticosterone, up to mildly supraphysiological levels, were found to have no effect on basal cartilage metabolism. These results suggest that physiological levels of glucocorticoids do not exert an inhibitory effect on the uptake of 35SO4(2-) into immature rat cartilage. Since butanol-soluble inhibitors of cartilage metabolism were found in adrenalectomized rat serum it is unlikely that these substances are glucocorticoids.
在大鼠软骨生长介素生物测定中，肾上腺切除大鼠的血清与未切除肾上腺动物的血清效力相当。用丁醇提取正常或肾上腺切除大鼠的血清，会降低其在生长介素生物测定中的效力，而不是像先前报道的那样提高效力。在正常和肾上腺切除大鼠的血清中均发现了可抑制软骨代谢的丁醇溶性物质。发现皮质醇和皮质酮在高达轻度超生理水平时，对基础软骨代谢没有影响。这些结果表明，糖皮质激素的生理水平不会对35SO4(2-)进入未成熟大鼠软骨产生抑制作用。由于在肾上腺切除大鼠血清中发现了可抑制软骨代谢的丁醇溶性物质，所以这些物质不太可能是糖皮质激素。
