Johnson R C, Shah S N
J Neurochem. 1981 Sep;37(3):594-6. doi: 10.1111/j.1471-4159.1982.tb12527.x.
In the present study, the properties, such as pH optima, detergent requirement, and effects of various lipids, of cholesterol ester hydrolase in human cerebrospinal fluid (CSF) were examined, and the activity levels of the enzyme in CSF from multiple sclerosis (MS) patients and non-MS individuals were compared. Our data indicate that the enzyme in CSF exhibits two pH optima: pH 6.0 in the presence of Triton X-100 and pH 7.0 in the presence of sodium taurocholate. Both phosphatidylethanolamine (PE) and phosphatidylserine (PS) enhanced the hydrolase activity at pH 6.0. The activity at pH 7.0, on the other hand, was enhanced slightly in the presence of PE but was inhibited in the presence of PS. These data suggest the presence of two cholesterol ester hydrolases in CSF and also indicate that the activity at pH 6.0 may be due to microsomal enzyme in brain and that at pH 7.0 may be due to myelin enzyme. The hydrolase activity at pH 7.0 was significantly lower in CSF from MS patients. The activity at pH 6.0 in CSF from MS and non-MS patients, however, did not differ significantly. This indicates that the reduction in pH 7.0 hydrolase activity in CSF may be related to demyelination.
在本研究中，检测了人脑脊液（CSF）中胆固醇酯水解酶的特性，如最适pH值、去污剂需求以及各种脂质的影响，并比较了多发性硬化症（MS）患者和非MS个体脑脊液中该酶的活性水平。我们的数据表明，脑脊液中的该酶表现出两个最适pH值：在Triton X - 100存在下为pH 6.0，在牛磺胆酸钠存在下为pH 7.0。磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）在pH 6.0时均增强了水解酶活性。另一方面，在pH 7.0时，PE存在时活性略有增强，而PS存在时活性受到抑制。这些数据表明脑脊液中存在两种胆固醇酯水解酶，也表明pH 6.0时的活性可能归因于脑中的微粒体酶，而pH 7.0时的活性可能归因于髓磷脂酶。MS患者脑脊液中pH 7.0时的水解酶活性显著较低。然而，MS患者和非MS患者脑脊液中pH 6.0时的活性没有显著差异。这表明脑脊液中pH 7.0水解酶活性的降低可能与脱髓鞘有关。
