Shinomiya M, Matsuoka N, Shirai K, Saito Y, Kumagai A
Atherosclerosis. 1979 Jul;33(3):343-50. doi: 10.1016/0021-9150(79)90185-0.
Cholesterol esterase activity was estimated in homogenates of rat arterial wall using radioactive cholesteryl oleate incorporated into phospholipid vesicles as a substrate. The labeled oleic acid was separated from the ester by addition of benzene-chloroform-methanol mixture. Under these conditions, two pH optima were found at about 4.5 and 7.5. Most of the activities at pH 4.5 and 7.5 were found in the lysosomal and microsomal fraction, respectively. No enzyme activity was detected when the substrate vesicles were prepared with phosphatidylethanolamine or sphingomyelin, but the activity was higher when the substrate vesicles were prepared with phosphatidylserine and highest when they were prepared with phosphatidylcholine. The relationship between enzyme regulation and lipid deposition in the arterial wall is discussed.
使用掺入磷脂囊泡中的放射性胆固醇油酸酯作为底物，在大鼠动脉壁匀浆中估计胆固醇酯酶活性。通过添加苯 - 氯仿 - 甲醇混合物将标记的油酸与酯分离。在这些条件下，发现两个pH最佳值分别约为4.5和7.5。在pH 4.5和7.5时的大部分活性分别存在于溶酶体和微粒体部分。当用磷脂酰乙醇胺或鞘磷脂制备底物囊泡时未检测到酶活性，但当用磷脂酰丝氨酸制备底物囊泡时活性较高，而用磷脂酰胆碱制备时活性最高。讨论了酶调节与动脉壁脂质沉积之间的关系。
