Bradshaw J M, Downing S J, Moffatt A, Hinton J C, Porter D G
J Reprod Fertil. 1981 Sep;63(1):145-53. doi: 10.1530/jrf.0.0630145.
Relaxin was extracted from the ovaries of pregnant rats. Material possessing uterine relaxing activity in vitro was eluted in three peaks from Sephadex G.50 columns. The 'G3 peak' material eluting in a position comparable to that of porcine relaxin inhibited myometrial activity of rats in vivo, improved the rate of rise of pressure of intrauterine pressure cycles in vivo, and, when administered to rats following progesterone and oestrogen priming, increased the distensibility of the cervix in vitro. This material also stimulated inter-pubic ligament formation in the mouse.. This rat relaxin material therefore exhibits biological actions in the rat similar to those previously assigned to porcine relaxin.
松弛素是从怀孕大鼠的卵巢中提取的。具有体外子宫舒张活性的物质从葡聚糖G-50柱上以三个峰洗脱下来。在与猪松弛素相当的位置洗脱的“G3峰”物质在体内抑制大鼠子宫肌层活性，提高体内子宫内压周期的压力上升速率，并且在给予孕酮和雌激素预处理后的大鼠时，在体外增加宫颈的扩张性。这种物质还刺激小鼠耻骨间韧带的形成。因此，这种大鼠松弛素物质在大鼠中表现出的生物学作用与先前归因于猪松弛素的作用相似。
