人型支原体在人二倍体细胞培养系统中的行为

Behavior of Mycoplasma hominis in a human diploid cell culture system.

作者信息

Sasaki T, Shintani M, Yamada K, Okumura H, Kihara K

出版信息

Microbiol Immunol. 1981;25(6):537-43. doi: 10.1111/j.1348-0421.1981.tb00055.x.

DOI:10.1111/j.1348-0421.1981.tb00055.x

Abstract

The behavior of Mycoplasma hominis in normal human embryonic lung fibroblast (HAIN-55) cell cultures was investigated. Multiplication patterns of cell-associated mycoplasmas and of extracellular mycoplasmas in the HAIN-55 cultures depended upon the size of the inoculum. This relationship did not vary with the number of days the cells had been cultured, nor with the number of HAIN-55 cell passages. The maximum mycoplasmal growth was obtained with inoculum sizes of 10(5) to 10(6) colony-forming units (CFU)/ml. The recovery of mycoplasmas decreased rapidly with inoculum size beyond 10(7) CFU/ml, and growth of the HAIN-55 cells was inhibited. Growth of the cells was also inhibited by the addition of the cytoplasmic fraction of Mycoplasma hominis.

摘要

研究了人型支原体在正常人胚肺成纤维细胞（HAIN - 55）培养物中的行为。HAIN - 55培养物中与细胞相关的支原体和细胞外支原体的增殖模式取决于接种物的大小。这种关系并不随细胞培养的天数或HAIN - 55细胞传代次数而变化。接种量为10⁵至10⁶菌落形成单位（CFU）/ml时可获得最大支原体生长。接种量超过10⁷CFU/ml时，支原体回收率迅速下降，且HAIN - 55细胞的生长受到抑制。添加人型支原体的细胞质部分也会抑制细胞生长。