Furness G, Whitescarver J, Trocola M, DeMaggio M
J Infect Dis. 1976 Sep;134(3):224-9. doi: 10.1093/infdis/134.3.224.
The morphology of viable Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma orale types 1 and 2, and Mycoplasma salivarium was studied in broth cultures by interference microscopy and in thin sections by electron microscopy. Only spherical cells were seen by interference microscopy. M. hominis had a capsule-like outer layer. Except for M. orale type 1, mycoplasmas in thin sections were 0.3-1 mum in diameter, with a bounding trilaminar membrane 7.5-10 nm thick. The mycoplasmas contained DNA fibrils and randomly distributed ribosomes. No polyribosomes were seen. Dividing mycoplasmas elongated slightly; the membrane invaginated, forming one bud. Sometimes M. hominis and M. salivarium produced one bud by elongation, and the bud was attached by a tube. This method of division is not considered as characteristic but rather as due to centrifugal force separating unfixed cells during preparation for electron microscopy. Cross-septa were never observed. In thin sections M. orale type 1 was elongated and without buds, an observation which suggested that preparation for electron microscopy distorted the mycoplasmas.
通过干涉显微镜对肉汤培养物中的发酵支原体、人型支原体、口腔支原体1型和2型以及唾液支原体的活菌体形态进行了研究，并通过电子显微镜对薄切片进行了观察。干涉显微镜下仅见球形细胞。人型支原体有一层类似荚膜的外层。除口腔支原体1型外，薄切片中的支原体直径为0.3 - 1μm，有一层厚7.5 - 10nm的三边界膜。支原体含有DNA纤维和随机分布的核糖体，未见多聚核糖体。正在分裂的支原体略有伸长；细胞膜内陷，形成一个芽。有时人型支原体和唾液支原体通过伸长产生一个芽，芽通过一根细管相连。这种分裂方式不被认为是其特征性的，而更可能是由于在电子显微镜样品制备过程中离心力使未固定的细胞分离所致。从未观察到横隔。在薄切片中，口腔支原体1型呈伸长状且无芽，这一观察结果提示电子显微镜样品制备过程使支原体发生了变形。
