Görsch R, Bergner H, Adam K
Arch Tierernahr. 1978 Jul;28(7):499-515. doi: 10.1080/17450397809436255.
Four Merino Landrace wethers averaging 47.6 kg body weight were adapted to a semi-synthetic diet containing as the only N-source 60 g of IBDU per day. After the adaptation phase, on the 1 st experimental day the IBDU of the morning feed was given in 15N-labelled form (701 mg 15N-excess). After 2 1/2, 7 1/4, 12 and 24 hours the experimental animals were killed without having been fed again. The comparison of the IBDU-concentrations in the content of the rumen bottom with the residual rumen content did not allow to draw conclusions regarding IBDU-sedimentation at the bottom of the rumen. For the 15N-decline in the rumen content, a relationship was established following y = 76.3 - 2.62 (r = 0.96) (see fig. 2). In the order of killing times the following 15N-IBDU amounts were retrieved (% of intake): I = 15.6%, II = 24.1%, III = 3.3% and IV = 3.6%. 7 1/4 hours after starting the experiment, 40% of the 15N-labelled material were found in the rumen in the form IBDU; after 12 hours it came to 10%. Except for sheep I, 15N-urea was not found but in small amounts. Only sheep I and III revealed IBDU-traces in the abomasum, but in the small intestine of all sheep 2 to 6% of the amount taken in. This fact is explained with the endogenous influx of IBDU from the blood. An additional experimental sheep provided with a ligature at the abomasum entry, revealed that IBDU is absorbed from the rumen and allowed to enter the individual segments of the intestine in small amounts.
四只平均体重为47.6千克的美利奴长白阉羊适应了一种半合成日粮，该日粮每天含有60克异丁基二脲（IBDU）作为唯一的氮源。适应期过后，在第1个实验日，早晨饲料中的IBDU以15N标记形式提供（701毫克15N过量）。在2.5、7.25、12和24小时后，实验动物未再次喂食即被处死。比较瘤胃底部内容物中的IBDU浓度与瘤胃残余内容物，无法得出关于IBDU在瘤胃底部沉降的结论。对于瘤胃内容物中15N的下降情况，建立了如下关系：y = 76.3 - 2.62（r = 0.96）（见图2）。按照处死时间顺序，回收的15N - IBDU量如下（占摄入量的百分比）：I = 15.6%，II = 24.1%，III = 3.3%，IV = 3.6%。实验开始7.25小时后，瘤胃中40%的15N标记物质以IBDU形式存在；12小时后降至10%。除了绵羊I外，未发现15N - 尿素，但有少量存在。只有绵羊I和III在皱胃中发现了IBDU痕迹，但在所有绵羊的小肠中发现了摄入量的2%至6%。这一事实可通过IBDU从血液中的内源性流入来解释。另一只在皱胃入口处结扎的实验绵羊表明，IBDU从瘤胃被吸收，并少量进入肠道的各个节段。
