Stoyanov I S, Chernozemsky I N, Nicolov I G, Petkova-Bocharova T, Stoichev I I
Oncodev Biol Med. 1980;1(4-5):291-300.
LDH isoenzymes were assayed by acrylamide disc electrophoresis in the following material: (a) cells from 4 transplantable hamster tumors; (b) cells from the same tumors cultured for 0, 4, 12 or 24 h as well as for 3, 7, 12 or 26 days; (c) tumors obtained after re-inoculation of cells cultured for 26 days. During the first days of culture, the cells retained LDH patterns similar to those of tumors in vivo. Then a progressive increase in the M/H ratio was observed in all cultures. This was followed by a complete disappearance of LDH 1,2,3. In the tumors appearing after re-inoculation of cultured cells, re-emergence of the patterns seen in the original tumors was noted. The mechanism of the observed changes remains hypothetical although an adaptive re-arrangement of gene activity controlling the production of M and H subunits and/or in vitro selection of clones with higher M/H ratio is postulated.
(a) 4种可移植性仓鼠肿瘤的细胞；(b) 来自同一肿瘤的细胞，分别培养0、4、12或24小时以及3、7、12或26天；(c) 接种培养26天的细胞后获得的肿瘤。在培养的最初几天，细胞保留了与体内肿瘤相似的乳酸脱氢酶模式。然后在所有培养物中观察到M/H比值逐渐增加。随后乳酸脱氢酶1、2、3完全消失。在接种培养细胞后出现的肿瘤中，注意到出现了原始肿瘤中所见的模式。尽管推测存在控制M和H亚基产生的基因活性的适应性重新排列和/或体外选择具有更高M/H比值的克隆，但观察到的变化机制仍然是假设性的。
