Miki K, Oda T, Miyazaki J, Iino S, Niwa H, Oka H, Suzuki S
Oncodev Biol Med. 1980;1(4-5):313-23.
Intestinal metaplasia and carcinoma of the stomach were produced in Wistar strain rats by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Alkaline phosphatase (AlP) isoenzymes of tissues from intestinal metaplastic mucosa and carcinoma of the stomach were studied. The ALP activity from all carcinoma tissues was several times higher than that of the surrounding gastric mucosa, and two of the tissues has over 100 times higher activity. An AlP zymogram from carcinoma tissues of the stomach showed one broad active band on 5% polyacrylamide-gel disc electrophoresis. This band was separated into 2 sharp active bands after treatment with neuraminidase: A-band (Rf = 19%) and B-band (Rf = 32%). From the enzymological and immunological study, B-band had properties similar to those of AlP isoenzyme from the intestinal metaplastic mucosa of the stomach, as well as intestinal AlP isoenzyme, while A-band had different ones. Anm AlP zymogram from glandular mucosa of the stomach showed 2 types of active band (A and B band) after treatment with neuraminidase, which were identical with those appearing in carcinoma tissues. The properties of AlP isoenzyme from the intestinal metaplastic mucosa of rat stomach induced by MNNG were similar to those of humans, and the carcinoma tissues of rat stomach were suspected of producing high amounts of AlP, especially the intestinal-type AlP isoenzyme.
通过口服N-甲基-N'-硝基-N-亚硝基胍（MNNG）在Wistar品系大鼠中诱发胃的肠化生和癌。对肠化生黏膜和胃癌组织中的碱性磷酸酶（AlP）同工酶进行了研究。所有癌组织的AlP活性均比周围胃黏膜的活性高几倍，其中两个组织的活性高出100倍以上。胃癌组织的AlP酶谱在5%聚丙烯酰胺凝胶圆盘电泳上显示出一条宽的活性带。用神经氨酸酶处理后，这条带被分离成两条清晰的活性带：A带（Rf = 19%）和B带（Rf = 32%）。从酶学和免疫学研究来看，B带具有与胃肠化生黏膜的AlP同工酶以及肠AlP同工酶相似的性质，而A带则不同。胃腺黏膜的AlP酶谱在用神经氨酸酶处理后显示出两种活性带（A带和B带），与癌组织中出现的带相同。由MNNG诱发的大鼠胃肠化生黏膜的AlP同工酶性质与人类相似，并且怀疑大鼠胃癌组织会产生大量的AlP，尤其是肠型AlP同工酶。
