Davey D F, O'Brien G M
Aust J Exp Biol Med Sci. 1978 Aug;56(4):409-19. doi: 10.1038/icb.1978.46.
Excised rat extensor digitorum longus muscles were soaked in a Krebs solution made hypertonic with extra NaCl and fixed using a combination of acrolein and glutaraldehyde dissolved in the same hypertonic Krebs. Electron microscopic examination showed that the sarcoplasmic reticulum (S.R.) of these muscles occupied the same fraction of fibre volume as it did in control muscles soaked and fixed in normal Krebs solution, contradicting previous studies on frog muscle. It appears unlikely that the S.R. is the rapidly exchanging Na+-compartment of rat muscle which enlarges in hypertonic solutions, or that it is an extracellular compartment. The T-system of hypertonic muscles was swollen, but its volume is insufficient to form the rapidly exchanging Na+-compartment.
切除的大鼠趾长伸肌浸泡在添加额外氯化钠使其变为高渗的 Krebs 溶液中，并使用溶解在相同高渗 Krebs 溶液中的丙烯醛和戊二醛混合物进行固定。电子显微镜检查显示，这些肌肉的肌浆网（S.R.）在纤维体积中所占比例与浸泡并固定在正常 Krebs 溶液中的对照肌肉相同，这与之前对青蛙肌肉的研究结果相矛盾。肌浆网似乎不太可能是大鼠肌肉中在高渗溶液中会扩大的快速交换 Na⁺ 区室，也不太可能是细胞外区室。高渗肌肉的 T 系统肿胀，但其体积不足以形成快速交换 Na⁺ 区室。
