支链氨基酸对用丙氨酸脱氢酶测定丙氨酸的干扰。
Interference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.
作者信息
Lund P, Baverel G
出版信息
Biochem J. 1978 Sep 15;174(3):1079-82. doi: 10.1042/bj1741079.
Abstract
Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.
摘要
枯草芽孢杆菌丙氨酸脱氢酶的商业制剂在不同程度上被对支链氨基酸的活性所污染。这些氨基酸的米氏常数与L-丙氨酸的米氏常数处于同一数量级（约10^(-3)M）。支链氨基酸脱氢酶活性在对水或2mM乙二胺四乙酸透析2至4小时后丧失。
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